Journal: Biomicrofluidics

Article Title: Embedding objects during 3D printing to add new functionalities

doi: 10.1063/1.4958909

Figure Lengend Snippet: (a) 3D CAD model of the first optical device without the fiber. (b) Schematic diagram of the exploded cross-sectional view of Section A–A depicted in (a) with the fiber. The first 3D printed optical device with embedded Corning® Fibrance™ Light-Diffusing Fiber (c) was not and (d) was lit up using a green laser pointer.

Article Snippet: The first optical device was 3D printed with the Corning ® Fibrance™ Light-Diffusing Fiber embedded during 3D printing (Figs. ).

Techniques:

Journal: Biomicrofluidics

Article Title: Embedding objects during 3D printing to add new functionalities

doi: 10.1063/1.4958909

Figure Lengend Snippet: (a) 3D CAD model of the second optical device without the fiber, and the top and the bottom rectangular glass slides. (b) Schematic diagram of the exploded cross-sectional view of Section A–A depicted in (a) with the fiber, and the top and the bottom rectangular glass slides. (c) The second 3D printed optical device with embedded Corning® Fibrance™ Light-Diffusing Fiber, and the integrated top and bottom 75 mm × 50 mm and 0.96 mm to 1.06 mm thick rectangular glass slides. (d) The device was lit up using a green laser pointer.

Article Snippet: The first optical device was 3D printed with the Corning ® Fibrance™ Light-Diffusing Fiber embedded during 3D printing (Figs. ).

Techniques:

Journal: Cell stem cell

Article Title: Human ESC-derived chimeric mouse models of Huntington disease reveal cell-intrinsic defects in glial progenitor cell differentiation

doi: 10.1016/j.stem.2018.11.010

Figure Lengend Snippet: Astrocytic differentiation was significantly delayed in mHTT glial chimeras. Mice neonatally transplanted with normal HTT GENEA19-derived GPCs began to develop significant donor-derived GFAP+ astrocytes by 8 weeks (A), robustly so by 13 weeks (B), with dense astrocytic colonization of the callosal white matter by 18 weeks (C). In contrast, mHTT-expressing hGPCs derived from GENEA20 sibling hESCs developed astrocytic phenotype more slowly, with little evident GFAP expression at 8 and 13 weeks (D and E), and only modest GFAP+ astrocytic maturation at 18 weeks (F). G-H, The mature astrocytic morphologies of mHTT-expressing and control astrocytes differed, in that mHTT astrocytes typically failed to manifest the degree of radial symmetry of their control-derived counterparts. I, The proportion of GFAP-expressing cells among all donor cells was consistently lower in mHTT hGPC-engrafted than control-engrafted mice. J-K, Sholl analysis of cells traced in NeuroLucida in 3D and shown flattened (O-P), revealed that normal donor astrocytes exhibited greater fiber complexity (J), more primary processes (K), yet shorter average and maximal fiber lengths (L-M), than mHTT-expressing astroglia. N-P, Fan-in radial analysis of volume occupancy (Dang et al., 2014) revealed that mHTT astrocytes had significantly more regions unoccupied by glial processes than did control astrocytes (N; illustrations in O and P), indicating their discontiguous domain structure.

Article Snippet: For the assessment and quantitative description of astrocytic fiber 3D architecture, Fan-in analysis (MBF Biosciences) was used as previously described for studies of dendritic topology ( Dang et al., 2014 ).

Techniques: Derivative Assay, Expressing, Control